Carbonic anhydrase under pressure

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Carbonic anhydrase under pressure

Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.

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Carbonic anhydrase in human platelets.

The carbonic anhydrase activity of human platelets was investigated by measuring the kinetics of CO2 hydration in supernatants of platelet lysates by using a pH stopped-flow apparatus. An average carbonic anhydrase concentration of 2.1 microM was determined for pellets of human platelets. Analysis of the kinetic properties of this carbonic anhydrase yielded a Km value of 1.0 mM, a catalytic-cen...

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Thermodynamics of Carbonic Anhydrase Catalysis

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ژورنال

عنوان ژورنال: IUCrJ

سال: 2018

ISSN: 2052-2525

DOI: 10.1107/s2052252517018012